Interleukin-1 gene variations in post-orthodontic external root resorption


Petra Linhartová (1, 2), Pavlína Černochová (2), Martina Raudenská (1), Jolana Lipková (1), Lydie Izakovičová Hollá (1, 2)


  1. Department of Pathophysiology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic
  2. Clinic of Stomatology, Institutions Shared with St. Anne's Faculty Hospital, Faculty of Medicine, Masaryk University, Pekařská 664/53, 602 00 Brno, Czech Republic.

External apical root resorption (EARR) is a permanent shortening of the end of the tooth root. EARR is a common clinical complication of orthodontic treatment and is known to occur after trauma and in response to inflammation. Polymorphisms in the interleukin-1 (IL-1) genes play a role in the inflammatory diseases through the modulation of cytokine levels. The aim of this study was to associate two single nucleotide polymorphisms (SNPs) and one variable number of tandem repeats (VNTR) polymorphism in IL-1 with EARR in the Czech population. In this case-control association study, 32 patients with EARR (age 15.3 ± 5.4 years) and 76 controls (age 15.1 ± 4.0 years) were genotyped using methods based on PCR for IL-1 gene polymorphisms [IL-1α (-889C/T), IL-1β (+3953C/T), and IL-1RN (IL-1 receptor antagonist, VNTR)]. While no statistical significant association was found in the genotype and allele frequencies of IL-1α (-889C/T) and IL-1β (+3953C/T) between patients with EARR and controls, marginally significant differences were observed in the genotype and allele frequencies of IL-1RN (p = 0.08 and p = 0.07, respectively). Highly significant differences of genotype and allele distributions were identified between patients with EARR and controls in the subgroup of women (p = 0.004 and p = 0.02, respectively). Although no significant role of IL-1α (-889C/T) and IL-1β (+3953C/T) variants in EARR was confirmed, our results suggest that IL-1RN VNTR may be associated with EARR, especially in women.
This study was supported by projects 1M0528 and IGA NT11405-6.

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